On Bottom Target — ASC — Tubeworm Pillar (X = , Y= )

Bottom Targets                                  X                                                        Y

M vent                                                4993                                                   77708

Bio 9                                                  4612                                                   78026

TICA                                                  4597                                                   78211

P-vent                                                4623                                                   77976

Bio 9                                                   4612                                                   78026

Alvinella Stump                                4612                                                   78026

Bio Marker 89 (Ty, IO)     

Tubeworm Pillar  
                                                               

DIVE OBJECTIVES BRIEF:

Primary Objective —Reconnaissance of Alvinella sites along transect, sites for ASU chimney unit deployments, and sampling of snow blower features.

1) Locate Tubeworm Pillar — survey for Alvinella/small chimneys.

2) If locate Alvinella, take temp./sample — place in cold box.

3) If small chimney, take temp.–collect.

4) Begin transit to Marker 82 and Ty and Io — survey Alvinella.

5) Take temps/sample if available.

6) Survey Alvinella Pillar.

7) Transit to P vent and Bio9 — survey, take temps., collect.

8) Transit to TICA — survey, take temps.

9) Transit to Q vent — survey, take temps.

10) Find small beehive to deploy ASU frying pan.

11) Transit to M vent and Robin's Roost — survey, take temps.

Detailed Instructions:

1) On descent, review  still and video (hand-held and sub) camera operation.

2) Review Nav and target dropping procedure.

3) Test Sipper and temp. probe — ASM?

4) WHITE BALANCE VIDEO.

5) Land west of ASC at latitude of Tubeworm Pillar.

6) Move in and survey all Alvinella colonies from TWP to M vent.

7) Using either Hi-Temp. probe or SIPPER probe, take temps. — RECORD.

8) Survey for small chimney/beehive features — get temp/XY.

9) Survey for snow blowers — take AMS samples — get XY.

1) Examine Alvinella clump (150°C)  for  large accessible tubes w/worms.

2) Take internal temp. of several tubes, record on dive sheet. The avg. temp. inside tubes should be 65°C or greater.  If not, note and proceed.

3) If hot, take  temps. (X6 tubes) and Sipper x4 (different tubes). Draw picture — note time and location on data sheet.

4) Position overhead camera so that it is looking down into DISSR. The other camera  should be recording Alvinella clump.

5)  Move a large clump to the top of the lg. Biobox.  If worms are right size ...

6) Open DISSR — remove plug.

7) Collect 4–6 large (6–8 med.) worms without damaging them. Avoid tube and debris — they take time to sink. Make sure they go down.

8) Use plug to encourage them to sink

9) Need good visuals of worms going into DISSR — note time.

10) Take good still shot/video of worm going into box for Ambion – NOTE.

11) Close DISSR — note on dive summary report sheet.

Alvinella — other:

12) Collect additional Allvinella clump and place in middle Biobox (COLD).

13) Collect a few more Alvinella and place into forward-most Biobox.

14) Take visuals and temps. if necessary.

15) Visualize number collected. BUT DON'T BE GREEDY — if good we will want to return later.

Survey throughout transect for small beehive/chimneys for rest of dive series. Where possible, take temps. and get good video — note time and XY.

16) Find a respectable beehive in the Q vent area for frying pan deployment.

17) Take temp. — top, side, and Bottom. Take SIPPER side and top  — record sample positions on data sheets.

18) Want temp 150–200°C — but not higher.

19) Collect — and place in rear of lg Biobox. Try not to knock down chimney.

20) Take temp. at scar — take Sipper if appropriate temp.

21) Place frying pan on scar — let sit 5 mins. Retake temp. and Sipper.

Snow Blowers:

1) Keep a constant eye out for snow blowers.

2) If locate, position for AMS sampling.

3) Take 1–3 samples from site — with good visuals — still and video.

4) Note XY, heading, time, etc., on data sheet and photo sheet.

5) When surfacing, take remaining AMS samples at intervals.