On bottom target — Bio-9 (X=4612, Y=78026 )

Bottom Targets                     X                                   Y

M vent                              4993                              77708

Q vent                              4447                              78847

P-vent                              4623                              77976

P-vent (sulfur spire)    4671                              77892

Fish Hole                        4652                              77912

Neo-Fish Hole               4660                              77951.6 314

Bio 9                               4612                              78026

TICA                               4597                              78211

Primary Objectives: Arrive at BIO-9 — take Sipper/chem. and collect large sulfide with Alvinella worms, collect hot worms if possible, collect Mosquito, move to TICA and deploy Cavanaugh samplers, move to East Wall for small Riftia collection in Shank sampler, one large Riftia in Biobox, move to Q vent and collect Alvinella at old Frying Pan site with Sippers, DISSR, and right compartment, collect large piece of chimney for rock samples.

FOR ALL SIPPER SAMPLES — DON’T FORGET TO TURN PUMP ON!!!!!!!!

Alvinella:

Take temp. of several tubes with Sipper wand — if over 50°C take Sipper chem.

Collect worms — place into biobox or DISSR.

Take temp. and chem. of bare sulfide surface where tubes were collected.

Beehive/chimney:

Take temp. and Sipper chem. of beehive on side and top.

If feature has orifice, do not put Sipper in it — take temp./chem. on side.

Collect beehive and place in lg. Biobox.

ASU Recovery (Frying Pan, Foreplay, Arnold).

Take temp. at unit.

Take Sipper/chem.

Remove ASU unit from sulfide.

Place in coffin.

Dive Objectives:

While descending, check Sipper, temp. probe for function.

Move into Bio-9.

Move to top of main Spire.

Take Sipper temp. and chem. X2. Use bottles 1,2,9,10.

Collect spire and place into large section of Biobox.

Move in to do Alvinella sampling on spire if worms are there, temp. is right, and it is possible. If not, wait for Q-vent.

Locate hot Alvinella.

Position for temp. survey — use Sipper probe if working — if not use Hi-T.

Take Sipper sample/temp. from inside tubes — use bottles 3,4, 11,12. Get good visuals (video and still) if temps are above 50°C.

Take your time here — may be best to grab clump and shake above DISSR.

Collect Alvinella and place onto Coffin lid — sort for DISSR.

Collect Alvinella and place into DISSR.

Try suction method for worms on sulfide.

Use plunger to push samples down into RNAlater.

Close lid.

Collect large clump of hot (65°C) Alvinella place into right-most chamber of lg. Biobox. Try not to collect sulfides with worms. Use camera to get idea of number and size.

Take Sipper of sulfide surface (use bottle 5).

Collect Mosquito.

Place in Biobox with chimney.

Take Sipper — chem. and temp. in hole (use bottle 6).

Go to TICA.

Place 3 Cavanaugh collectors at 10’ intervals in line from clump.

Place last unit 90° to right or left of fourth unit — get (X= Y= , and headings).

Look for small Riftia at base.

Place a small clump into Shank unit.

Grab a medium worm; de-tube and cut 2/3 down.

Let bleed a moment — place into Shank sampler.

Put in Shank stopper — FIRE.

Move up to Q-vent.

Locate Frying Pan location and collect Alvinellas as above.

Collect large piece of new hard chimney for rock collection.

Place in milk crate.

On ascent — please place manipulators on coffins if possible.

Request DBL NAV screen shot at end of dive.

On descent, review still and video (hand-held and sub) camera operation.

Review Sipper/AMS operations.

Land EAST of ASC at latitude of Q-vent.

 1. Run Sipper temp. and chem. — X2 (charged and uncharged).

 2. Remove upper 18" of chimney and place into lg. section of Bio-box.

 3. Look to see if it is possible to sample Alvinellas there — if not wait for Q-vent.

Collection of Alvinella can be made at P-vent or Bio-9.

We are going to do chem. and temp. work on Alvinella — but we really want Hot guys (lots of shimmering water). Please take lots of pics of each procedure.

This can be done at Bio-9.

Take hand-held video/still of Alvinella before sampling. Position 3-chip to get some good video of the worms moving in and out of tube and sample area.

Take internal temp. of several tubes; record on dive sheet. The avg. temp. inside tubes should be 65°C (45°C outside) or greater.

Use the Sipper temp. probe and record data from overlays on data sheets.

If hot, take temps. in tubes (X3 tubes) and Sipper x3 (different tubes). Draw picture of positions — note time and location on data sheet.

b. Alvinella — DISSR.

IN ALL CASES WE NEED TO HAVE TEMP. AND SIPPER DATA FROM EACH COLONY SAMPLED.

Position overhead camera so that it is looking down into DISSR Sampler. The other camera should be recording Alvinella clump.

Move a clump to the top of the coffin — sort and drop animals into DISSR. Look to make sure you have worms — 10 – 20.

Collect 4–6 large (6–8 med.) worms without damaging them — avoid tube and debris if possible — they take time to sink. Make sure they go down.

Need good visuals of worms going into sampler — note time.

Take good still shot/video of worm going into box for Ambion — NOTE.

c. Collect mass Alvinella clump.

Collect lg. Alvinella clump and place in single chamber of Biobox with ice (RIGHT).

See if you can get a good clump without sulfide.

Visualize number collected.

Repeat procedure until you have a good number of worms in box (20–30).

Take final Sipper and temp. on the surface of the sulfide from where the worms were sampled.

Note on dive summary report sheet XY and headings. 

d. Collect Mosquito probe.

 1) Take Sipper/chem. in same hole as Mosquito.

Move to TICA

Deploy 3 Cavanaugh samplers.

Place one 10’ down strike from unit placed there yesterday.

Get XY and heading.

Place 4th 5–10 feet away down ASC.

Get XY and heading.

Place 5th unit 90° to East or West of 4th unit.

Get XY and heading.

Find a small clump of small Riftia.

Place into Shank sampler.

Collect one medium worm — cut 2/3 down and let bleed.

Place into Shank sampler — cap and fire.

Sample — as above.