Sub deployment location — ASC Q vent  9°50.78'N  104° 17.58'W  (just east Q vent)

Basket change: Basket will be rearranged slightly — with, SIPPER, large Biobox, DISSR, Shank sampler, milk crate, small Cary Biobox,  2X Cavanaugh samplers (1 lb. each), slurp pump.

Bottom Targets                                  X                                                        Y

M vent                                               4993                                                   77708

Q vent                                               4447                                                   78847

P vent                                               4623                                                   77976

P vent (sulfur spire)                           4671                                                   77892

Fish Hole                                          4652                                                   77912

Neo-Fish Hole                                  4660                                                   77951.6        

314 Bio-9                                         4612                                                   78026 TICA                                               4597                                                   78211

BRIDGE/DECK — We thank you for all your hard work.  With the loss of the Elevator, our deployments will come to a sad end.  I will consult with the CTD people but anticipate that if last night's operation went well, no more CTD deployments are planned.

Primary Objective — Collect ASU For play and Frying Pan, take SIPPERS of each, Alvinella collection at Q vent or M vent, collect beehive/chimney, pick up nice piece of chimney with chalcopyrite (gold) for ship-wide distribution, move to TICA — deploy Cavanaugh samplers.

Sampling procedure:

FOR ALL SIPPER SAMPLES — DON'T FORGET TO TURN PUMP ON!!!!!!!!

Alvinella:

1) Take temp. of several tubes with Sipper wand — if over 50°C take Sipper chem.

2) Collect worms — place into Biobox or DISSR.

3) Take temp. and chem. of bare sulfide surface where tubes were collected.

Beehive/chimney:

1) Take temp. and Sipper chem. of beehive on side and top.

2) If feature has orifice, do not put Sipper in it — take temp./chem. on side.

3) Collect beehive and place in lg. Biobox.

ASU deployment (Frying Pan, Foreplay, Arnold) — could be on beehive scar.

1) Take temp. — make sure between 150–250°C

2) Take Sipper/chem. just on sulfide.

3) Place ASU unit onto sulfide.

Take temp. and Sipper chem. of water coming through unit.

Dive Objectives:

1) While descending, check Sipper, AMS, and  temp. probe for function.

2) Move to Ty/Io.

3) Locate beehive/chimney feature or hot sulfide.

4) Take HiT temp. at orifice.

5) Take Sipper chem. and temp. at side in two places.

6) Collect chimney and place into far left compartment of Biobox.

7) Move to P-Vent — run slurp and AMS (x3) on side of chimney.

8) Move to Bio-9 for Alvinella collection.

9) Locate chimney with hot Alvinella — Blee said southern-most spire. Take  (X =   Y =  , Heading =  )

10) Position for temp. survey —use Sipper to make sure tubes are in excess of 50°C.

11) Take Sipper sample/temp. from inside tubes — X 3–6. Get good visuals (video and still) if temps. are above 50°C.

12) Collect large clump of hot (65°C) Alvinella. Place into right-most chamber of lg. Biobox with ice. Try not to collect sulfides with worms. Use camera to get idea of  number and size — we need 20–30 worms.

13) Collect more Alvinella and place into DISSR and Shank sampler. Close and secure DISSR.

14) Place plug into Shank sampler and fire unit — watch for rising plunger.

15) Confirm with Pan-and-Tilt that 6–8 worms are in sampler.

16) Collect another small clump and place into middle chamber of coffin.

17) Inspect Mosquito — (X=  , Y= , Heading  )

18) Move to LVWS site (X = 4653 , Y = 77953, heading = 322). Inspect Harold sampler.

19) If see filamentous strand growing — take AMS (X 2).

20) Go to Neo-Fish Hole, retrieve Harol, and move to high-flow site near Riftia.

21) If time permits — locate more Alvinella for temp. survey for next-day collection.

22) If time permits — go to Q and survey other two ASU units.

Request DBL NAV screen shot at end of dive.

1)    On descent, review still and video (hand-held and sub) camera operation.

2)    Review SIPPER/AMS operations.

3)    Land EAST of ASC at latitude of  Bio-9 vent.

A. Collect beehive or black chimney sample:

1)    Take 2 temp. and Sipper chem. of the SIDE of the chimney  — not in orifice.

2) Place into left chamber of Biobox.

1) Slurp chimney.

2) Fire 2 AMS against chimney.

c) Move to Bio-9.

We are going to do chem. and temp. work on Alvinella — but we really want Hot guys (lots of shimmering water).  Please take lots of pics of each procedure.

SIPPER INSTRUCTIONS:

At this stage it is really simple — the pilot does most of the work.  The most important thing to remember is that PRIOR TO EACH SAMPLE YOU MUST PUMP FOR 1 MIN. — then take sample.

1) Set up and do some preliminary temp. checks to make sure temps. are high (>50°C) at high-temp. site — Blee said there were a lot of Alvinellas on the southern-most spire about 5 meters up.

1) Take hand-held video/still of Alvinella before sampling. Position 3-chip to get some good video of the worms moving in and out of tube and sample area.

2) Take internal temp. of several tubes, record on dive sheet. The avg. temp. inside tubes should be 65°C (45°C outside) or greater.

3) Use the Sipper temp. probe and record data from overlays on data sheets.

4) If hot. take  temps. in tubes(X4 tubes) and Sipper x4 (different tubes). Draw picture of positions — note time and location on data sheet.

5) Collect lg. Alvinella clump and place in single chamber of Biobox with ice (RIGHT).

6) See if you can get a good clump without sulfide.

7) Visualize number collected.

8) Repeat procedure until you have a good number of worms in box (20–30).

9) Collect a small clump of Alvinella and place into middle compartment in lg. Biobox.

10) Take Final Sipper and temp. on the surface of the sulfide from where the worms were sampled.

IN ALL CASES, WE NEED TO HAVE TEMP. AND SIPPER DATA FROM EACH COLONY SAMPLED.

11) Examine Alvinella clump or large accessible tubes w/worms possibly same clump as above. Could be same colony as before if large enough.

12) Take internal temp. of several tubes; record on dive sheet. The avg. temp. inside tubes should be 65°C or greater.  If not, note and proceed.

13) If hot, take temps. (X6 tubes) and Sipper x4 (different tubes). Draw picture — note time and location on data sheet. 

14) Position overhead camera so that it is looking down into DISSR Sampler. The other camera  should be recording Alvinella clump.

15) Move a small clump to the top of the coffin — sort and drop animals into DISSR. Look to make sure you have worms — 6–10.

16) Collect 4–6 large (6–8 medium) worms without damaging them — avoid tube and debris if possible. They take time to sink. Make sure they go down.

17) Need good visuals of worms going into sampler — note time.

18) Take good still shot/video of worm going into box for Ambion — NOTE on dive summary report sheet XY and headings.

1) Inspect Harold sampler.

2) If has filamentous bacteria growing on it...

3) Take 2 AMS sample and temp.

1) Transfer Harold trap to high-flow site near to Riftia or Alvinella

I. Photo Doc. ASU Frying Pan and Foreplay currently in position.

Finally — if time and power allow — steam to Q-vent and survey/photo ASU Frying Pan. Take temp. if you have time.  (X=4469  Y= 78762 , heading = 132.5).  If you have a lot of time — get a temp.