On Bottom Target — ASC BIO-9 (9°N 50.335', 104°W 17.476')(X=4605, Y=78048)

M vent                              4993                              77708

Q vent                              4447                              78847

P-vent                              4623                              77976

P-vent (sulfur spire)     4671                              77892

Fish Hole                         4652                              77912

Neo Fish Hole                 4660                              77951.6 314

Bio 9                                 4612                              78026

TICA                                  4597                              78211

Dive Objectives Brief:

Primary Objectives: Release of LVWS, Alvinella collection, deploy Mosquito, ASU Frying Pan deployment at Ty/Io, ASU Arnold deployment at Ty/Io, collect beehive/black chimney, if possible travel to Q vent, fly-by of ASU Frying Pan, and Foreplay at Q vent if time permits.

Sampling procedure:

Alvinella

Take temp. of several tubes with Sipper wand — if over 50°C take SIPPER chem.

Collect worms — place into Biobox or DISSR.

Take temp. and chem. of bare sulfide surface where tubes were collected.

Beehive:

Take temp. and Sipper chem. of beehive on side and top.

If feature has orifice, do not put Sipper in it — take temp./chem. on side.

Collect beehive and place in lg. Biobox.

ASU deployment (Frying Pan, Foreplay, Arnold) — could be on beehive scar:

Take temp. — make sure between 150–250°C.

Take Sipper/chem. just on sulfide.

Place ASU unit onto sulfide.

Take temp. and Sipper chem. of water coming through unit.

Dive Objectives:

While descending, check Sipper and temp. probe for function.

Release Elevator

Move to Ty/Io.

Look for suitable site for ASU Frying Pan — 150–250°C.

Locate beehive feature or hot sulfide on relatively flat area for ASU Frying Pan deployment (150–250 °C temp.).

Take temperatures of area first — take Sipper, then collect if it is a beehive.

Take temp. and Sipper of scar — make sure it is not too hot - <300°C.

If no beehive is available — deploy using same sampling-specific sampling strategy with Sipper before and after deployment.

Deploy Frying Pan — take temp. and Sipper from center orifice.

Deploy Arnold — in cooler area (15–200°C).

Move to Bio-9 for Alvinella collection.

Take Sipper before and after.

Locate chimney with hot Alvinella — Blee said southernmost spire. Take (X = Y = , Heading = )

Position for temp. survey — use Sipper to make sure tubes are in excess of 50°C.

Take Sipper sample/temp. from inside tubes — X 3-6. Get good visuals (video and still) if temps. are above 50°C.

Collect large clump of hot (65°C) Alvinella. Place into right-most chamber of lg. Biobox with ice. Try not to collect sulfides with worms. Use camera to get idea of number and size — we need 20–30 worms.

Collect more Alvinella and place into DISSR sample — close and secure.

Confirm with Pan-and-Tilt that 6–8 worms are in sampler.

Collect another small clump and place into middle chamber of coffin.

Deploy Mosquito — get temp./Sipper before deployment in high-temp. tube.

Take Sipper and temp. of the hole before deployment.

If you locate snowblower feature in any area, slurp flock area for 10 min. only.

If time permits — locate more Alvinella for temp. survey for next-day collection.

If time permits — go to Q and survey other two ASU units.

Request DBL NAV screen shot at end of dive.

 Detailed instructions:

On descent, review still and video (hand-held and sub) camera operation.

Review Sipper/AMS operations.

Land EAST of ASC at latitude of Bio-9 vent.

A. Free Vehicle deployment and release:

Locate LVWS sampler.

Take temperature at intake.

Replace funnel and tubing.

Secure main switch.

Release.

B. ALVINELLA

We are going to do chem. and temp. work on Alvinella — but we really want Hot guys (lots of shimmering water). Please take lots of pics of each procedure.

If push comes to shove —

 1) Set up and do some preliminary temp. checks to make sure temps. are high (>50°C) at high-temp. site — Blee said there were a lot of Alvinellas on the southern-most spire about 5 meters up.

Take hand-held video/still of Alvinella before sampling. Position 3-chip to get some good video of the worms moving in and out of tube and sample area.

Take internal temp. of several tubes; record on dive sheet. The avg. temp. inside tubes should be 65°C (45°C outside) or greater.

Use the Sipper temp. probe and record data from overlays on data sheets.

If hot, take temps. in tubes(X4 tubes) and Sipper x4 (different tubes). Draw picture of positions — note time and location on data sheet.

Collect lg. Alvinella clump and place in single chamber of Biobox with ice (RIGHT).

See if you can get a good clump without sulfide.

Visualize number collected.

Repeat procedure until you have a good number of worms in box (20–30).

Collect a small clump of Alvinella and place into middle compartment in lg. Biobox.

Take final Sipper and temp. on the surface of the sulfide from where the worms were sampled.

C. Alvinella — DISSR sampler.

IN ALL CASES, WE NEED TO HAVE TEMP. AND SIPPER DATA FROM EACH COLONY SAMPLED.

Examine Alvinella clump or large accessible tubes w/worms possibly same clump as above. Could be same colony as before if large enough.

Take internal temp. of several tubes; record on dive sheet. The avg. temp. inside tubes should be 65°C or greater. If not — note and proceed.

If hot, take temps (X6 tubes) and sipper x4 (different tubes). Draw picture — note time and location on data sheet.

Position overhead camera so that it is looking down into DISSR sampler. The other camera should be recording Alvinella clump.

Move a small clump to the top of the coffin — sort and drop animals into DISSR. Look to make sure you have worms — 6–10.

Collect 4–6 large (6–8 med.) worms without damaging them — avoid tube and debris if possible — they take time to sink. Make sure they go down.

Need good visuals of worms going into DISSR Sampler — note time.

Take good still shot/video of worm going into box for Ambion — NOTE on dive summary report sheet XY and headings.

D. ASU FRYING PAN DEPLOYMENT IN HIGH-TEMP. DIFFUSE-FLOW AREA.

Locate a high-temp. diffuse-flow area to deploy Frying Pan unit — must be able to support unit. This could be at Bio-9 or Ty/Io.

Looking for 150–250°C if possible.

Might be best to look for exiting beehive.

Take temperature of beehive but not in orifice — make drawing of position.

Take Sipper — be careful not to knock it down !!!!!!

Take picture/video during sampling.

Open large section of lg. Biobox slowly.

Remove Frying Pan unit and place to side.

Remove beehive and place into coffin.

Take temp. and Sipper of remaining beehive scar if temp. is still in range.

Place Frying Pan unit onto scar — take temp. and Sipper sample near to one of the chambers.

Note XY and HEADING.

If Beehive is not available — find hot flat sulfide surface to place unit.

E. Deploy Arnold:

For Arnold we are looking for a cooler sulfide surface (150–200°C) for deployment.

Deployment is similar to the Frying Pan.

Essential to get Sipper temp. and chem. before and after deployment.

F. Collect beehive or black chimney sample.

Take temp. and Sipper of the SIDE of the chimney — not in orifice.

Place into left chamber of Biobox.

G. Photo Doc. ASU Frying Pan and Foreplay currently in position.

Finally — if time and power allow — steam to Q-vent and survey/photo ASU Frying Pan. Take temp. if you have time. (X=4469 Y=78762, heading = 132.5 ). If you have a lot of time — get a temp.

 MAKE SURE AT END OF DIVE YOU ASK FOR A SCREEN PICTURE OF DBL NAV.